品牌 | Bio-Rad/伯樂 | 供貨周期 | 現貨 |
---|---|---|---|
主要用途 | 實驗室 | 應用領域 | 食品,化工,生物產業,能源,制藥 |
產品描述
一瓶封閉緩沖液,500毫升。所有western印跡只需要5分鐘的封閉
每個印跡-5分鐘每個印跡阻斷
五分鐘的阻斷緩沖液,適用于所有印跡。
EveryBlot阻斷緩沖液為所有蛋白質印跡提供5分鐘的阻斷和較大靈敏度,無論檢測方法如何。
Bio-rad伯樂封閉液試劑 12010020
功能和優點
減少非特異性抗體結合以減少背景,同時保持優異的敏感性
用于化學發光和熒光檢測
不含磷酸鹽緩沖液。非常適合與磷酸特異性抗體一起使用
5分鐘內完成封閉
Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots
competitor-everyblot-5 minute everyblot blocking
A five minute blocking buffer for ALL western blots.
EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.
Features and Benefits
Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity
For both chemiluminescent and fluorescent detection
Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies
Complete blocking in 5 minutes
Bio-rad伯樂封閉液試劑 12010020
使用建議
對于小型印跡,使用至少10 ml用于阻斷和抗體孵育步驟。對于中等大小的印跡,至少使用20毫升。
將塊加入膜中,攪拌孵育5分鐘
將第一抗體和第二抗體稀釋在全強度塊中,并在攪拌下孵育1小時
對于PVDF的熒光檢測,將SDS添加到第二抗體溶液中的0.02%。
Recommended Protocol
For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.
Add block to the membrane and incubate for 5 minutes with agitation
Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation
For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.
1610374 | PRECISION PLUS STD DUAL COLOR | 雙色預染蛋白marker | Bio-rad伯樂 |
1610394 | Precision Plus Protein Dual Color Standards 5 Pack | 5個包裝的雙色預染蛋白marker | Bio-rad伯樂 |
1610375 | PREC PLUS STD KALEIDOSCOPE | 藍色預染蛋白marker | Bio-rad伯樂 |
1620177 | IMMUN-BLOT PVDF MEM,26CMX3.3M | PVDF膜 | Bio-rad伯樂 |
1705060 | Clarity Western ECL Substrate, 200ml | 100ml裝Clarity化學發光液 | Bio-rad伯樂 |
1705061 | Clarity Western ECL Substrate, 500ml | 250ml裝Clarity化學發光液 | Bio-rad伯樂 |
1708890 | KIT,iSCRIPT cDNA SYNTHESIS,25R | iScript™ cDNA 合成試劑盒 | Bio-rad伯樂 |
1708891 | KIT,iSCRIPT cDNA SYNTHES,100R | iScript™ cDNA 合成試劑盒 | Bio-rad伯樂 |
TBS0201 | TUBE,STRIP-8,NATL 120 STRIPS | PCR8聯管,高位 | Bio-rad伯樂 |
TCS0801 | CAP STRIP-8,DOMED 130 STRIPS | PCR8聯管蓋 | Bio-rad伯樂 |
TCS0803 | OPTICAL,8-CAP STRIP,FLAT (120) | PCR8聯管蓋通用 | Bio-rad伯樂 |
TLS0801 | TUBE,STRIP-8,LO-PRO NAT 120/PK | PCR8聯管,低位 | Bio-rad伯樂 |
TLS0851 | TUBE,STRIP-8,LO-PRO WHT 120/PK | PCR8聯管,低位 | Bio-rad伯樂 |
HSP9601 | HSP-96,WHT/CLR 50/BX | Hard-Shell® 96 孔全裙邊 PCR 反應板,低位通明 | Bio-rad伯樂 |
HSP9655 | HSP-96,WHT/WHT 50/BX | Hard-Shell® 96 孔全裙邊 PCR 反應板,低位白色 | Bio-rad伯樂 |
MLP9601 | MULTIPLATE-96,NATL 25/BX | Multiplate96孔無裙邊PCR反應板,高位 | Bio-rad伯樂 |